Epub 2015 Jun 15. Drug Discov Today 20(4):450–457, Napoli C, Lemieux C, Jorgensen R (1990) Introduction of a chimeric chalcone synthase gene into petunia results in reversible Co-suppression of homologous genes in trans. Next-generation genome sequencing and sophisticated genome-wide functional genomics' methods have led to a significant increase in the identification of novel drug target candidates and understanding of the relevance of these genomic and molecular changes for the diseases. There are a number of exciting developments in this domain, spanning a diverse array of applications that range from the highly person… Please enable it to take advantage of the complete set of features! PLoS One 7:14, Buehler E, Khan AA, Marine S, Rajaram M, Bahl A, Burchard J, Ferrer M (2012b) siRNA off-target effects in genome-wide screens identify signaling pathway members. Functional genomics‐based analgesic drug repurposing Repurposing screens using novel molecular techniques such as reprogrammed nociceptor neurons currently shift the trend from target‐based to pathway‐based repurposing, supported by the inclusion of computational techniques and … Chemical genetic-based phenotypic screen reveals novel regulators of gluconeogenesis in human primary hepatocytes. Proc Natl Acad Sci U S A. 43.229.77.143. Early characterization of toxicity and efficacy would significantly impact the overall productivity of pharmaceutical RD and reduce drug candidate attrition and failure. Keywords: Genome Engineering, including Zinc-finger, TALEN and most recently CRISPR/Cas9, has become a powerful tool in the drug discovery pipeline. 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BMC Bioinformatics 11:1471–2105, Sander JD, Joung JK (2014) CRISPR-Cas systems for editing, regulating and targeting genomes. Addiction to oncogenes–the Achilles heel of cancer. 2014 Dec;19(10):1327-37. doi: 10.1177/1087057114548414. Nature 509:487–491, © Springer International Publishing Switzerland 2015. Nat Biotechol 32:577–582, Helming KC, Wang X, Wilson BG, Vazquez F, Haswell JR, Manchester HE, Kim Y, Kryukov GV, Ghandi M, Aguirre AJ, Jagani Z, Wang Z, Garraway LA, Hahn WC, Roberts CW (2014) ARID1B is a specific vulnerability in ARID1A-mutant cancers. USA.gov. Next-generation genome sequencing and sophisticated genome-wide functional genomics' methods have led to a significant increase in the identification of novel drug target candidates and understanding of the relevance of these genomic and molecular changes for the diseases. Nat Biotechnol 32:347–355, Sanjana NE, Shalem O, Zhang F (2014) Improved vectors and genome-wide libraries for CRISPR screening. Nat Biotechnol 21:635–637, Jinek M, East A, Cheng A, Lin S, Ma E, Doudna J (2013) RNA-programmed genome editing in human cells. We very much thank Anne Adams for her help with designing the figures. Methods Mol Biol 942:193–204, Wang T, Wei JJ, Sabatini DM, Lander ES (2014) Genetic screens in human cells using the CRISPR-Cas9 system. Functional genomics describes a field of biology that uses a range of approaches for assessing gene function with high-throughput molecular, genetic, and cellular technologies. Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. | | 3 The long-term success of functional genomics platforms in addressing these challenges will be driven by … However, … Next-generation genome sequencing and sophisticated genome-wide functional genomics’ methods have led to a significant increase in the identification of novel drug target candidates and understanding of the relevance of these genomic and molecular changes for the diseases. eCollection 2018. Applications of Functional Genomics for Drug Discovery SLAS Discov. NIH Epub 2015 May 22. Not affiliated Nature Methods 3:701–706, Birmingham A, Anderson EM, Reynolds A, Ilsley-Tyree D, Leake D, Fedorov Y, Baskerville S, Maksimova E, Robinson K, Karpilow J, Marshall WS, Khvorova A (2006) 3′ UTR seed matches, but not overall identity, are associated with RNAi off-targets. J Biomol Screen 18:1164–1185, Pelz O, Gilsdorf M, Boutros M (2010) Web cellHTS2: a web-application for the analysis of high-throughput screening data. Nat Med 20:251–254, Hendel A, Fine EJ, Bao G, Porteus MH (2015) Quantifying on- and off-target genome editing. Part of Springer Nature. Genes Dev 16:948–958, Patel AC (2013) Clinical relevance of target identity and biology: implications for drug discovery and development. Next-generation libraries for robust RNA interference-based genome-wide screens. As functional genomic tool for target identification, high-throughput gene silencing through RNA interference screening has become the established method. 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